By Alan A. Boulton, Glen B. Baker
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Additional info for Amines & Their Metabolites (NEUROMETHODS) (Neuromethods 2 Series I Neurochemistry)
Carlsson A , and Rosengren E. (1958) A method for the fluonmetrlc determmatlon of adrenaline and noradrenalme m tissues. Acta physlol stand 44, 273-292. , Carlsson A , and Rosengren E. (1959) Fluonmetrlc method for differential estimation of the 3-0-methylated derivatives of adrenaline and noradrenalme (metanephrme and normetanephrme) Clm Chum Acfa 4, 456457. , Pletscher A , Brodie B. , and Udenfriend S (1956) Identification and assay of serotonm m brain I. Pharmacol Exp Ther 117, 82-88 Boulton A.
1981) have found that a slight modification of the OPT assay gave better agreement with the HPLC-ED method. Westermk (1982) has compared values for DA obtained by measurement of fluorescence after ethylene diamme condensation, DOPAC and HVA measured by the fluorescence procedure of Westerink and Korf (1977), and 5-HIAA assayed as its OPT condensate with values for the same four compounds obtained using HPLC-ED. He found a good correlation between the two methods for DOPAC and HVA, a poorer correlation for DA, and an unacceptable agreement for 5-HIAA, with the fluorescence methods being suspect m the latter two cases.
1972) and tryptophan (TP) (Denckla and Dewey, 1967) have been measured using such a reaction. Maickel and Miller (1966) analyzed 5-HT and 5-methoxytryptamme followmg condensation with o-phthalaldehyde (OPT). Curzon and Green (1970) found that the sensitivity of this method for 5-HT and 5-HIAA was increased by the addition of cysteme. Korf and Valkenburgh-Sikkema (1969) used the OPT reaction to measure 5-HIAA in urine after first destroymg 5-HT with periodate. Welch et al. (1972) automated the analysis of 5-HT based on the OPT condensation reaction.